백서 두개골 결손모델에서 하이드록시아파타이트 입자로 입혀진 실크단백이 골재생에 미치는 영향
The Effect of Silk Fibroin Particles Coated with Hydroxyapatites on Bone Regeneration in the Rat Calvarial Defect Model
석현, 박용태, 김성곤, 진형준,
소속 상세정보
석현 ( Seok Hyun ) - 강릉원주대학교 치과대학 구강악안면외과학교실
박용태 ( Park Yong-Tae ) - 강릉원주대학교 치과대학 구강악안면외과학교실
김성곤 ( Kim Seong-Gon ) - 강릉원주대학교 치과대학 구강악안면외과학교실
진형준 ( Jin Hyung-Joon ) - 인하대학교 고분자신소재공학과
KMID : 0360120130350010013
Abstract
Purpose: This study evaluated the capability of bone formation of silk fibroin particles coated with hydroxyapatites (HA/SF), as bone graft material when put into the calvarial defect of rats.
Methods: Twenty Sprague Dawley rats were used for this study and round shaped defects were formed in the center of parietal bones (diameter: 8.0 mm). The defect was filled with (1) HA/SF (experimental group), or (2) left as a vacant space (control group). The animals were sacrificed at 4 or 8 weeks, postoperatively. The specimens were decalcified and stained with Masson’s trichrome for histomorphometric analysis.
Results: The average of new bone formation was $33.18{\pm}3.10%$ 수식 이미지 in the experimental group and $20.49{\pm}5.79%$ 수식 이미지 in the control group at 4 weeks postoperatively. That was $42.52{\pm}7 수식 이미지.74%$ 수식 이미지 in the experimental group and $25.50{\pm}7.31%$ 수식 이미지 in the control group at 8 weeks postoperatively. The difference between the groups was significantly higher at both 4 weeks and 8 weeks postoperatively (P<0.05).
Conclusion:The rat calvarial defect was successfully repaired by HA/SF graft. The HA/SF graft showed more new bone formation compared with the unfilled control.
키워드
Silk fibroin;Hydroxyapatite;Bone regeneration;Rat calvarial defect
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